Identification Of <i>Arabidopsis</i> genes associated with cold tolerance based on integrated bioinformatics analysis

Cold stress is a major environmental factor that limits plant growth and productivity. Plants have evolved various strategies to adapt these conditions. To better explain the mechanisms used survive challenges, we retrieved cold-responsive genes of Arabidopsis thaliana from Gene Expression Omnibus (GEO) database. The GEO raw data were normalized by quantile method, then differentially expressed (DEGs) under cold screened using robust rank aggregation (RRA) algorithm, including 261 upregulated 177 downregulated out more than 20,000 genes. Further, integrated bioinformatics analyses PUBMED, PANTHER, DAVID, STRING indicated DEGs involved in cellular response red light, negative regulation circadian rhythm, photoprotection, monosaccharide transport, acclimation, phosphate ion homeostasis, while associated with indole glucosinolate biosynthetic process, RNA splicing, water cell wall modification, loosening, homeostasis. Furthermore, up-regulated had about four times protein-protein-interactions (PPIs) down-regulated DEGs, identified Cytoscape software. qRT-PCR low-temperature-responsive protein 78 (LTI78), transducin family (SWA1), arginine methyltransferase 11 (PRMT11) performed validate outcome analysis. Our work will improve our knowledge might be targets for stress-resistance research.